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Objective To understand the clinical value on the detection of carcinoembryonic antigen mRNA (CEA mRNA), cytokeratin 19 mRNA (CK19 mRNA) and telomerase in blood for the monitoring of blood metastasis of lung cancer. Methods CEA mRNA and CK19 mRNA were detected by reverse transcriptase-nested primers-polymerase chain reaction, telomerase was detected by telomeric repeat amplification protocol-hybridism-enzyme-linked immunosorbent assay in peripheral blood. Results The positive rates of the three tumor markers in lung cancer group were much higher than the non-tumor group and the health group (P<0.001). The sensibility of CEA mRNA and telomerase were much higher than CK19 mRNA (P<0.01). The positive rates of the markers in TNM stages Ⅲ and Ⅳ were much higher than in stages Ⅰ (P<0.05 to P<0.01). Conclusion It had high value that detecting CEA mRNA, CK19 mRNA and telomerase in peripheral blood on the discovery of blood metastasis of lung cancer. Among them, the clinical value of CEA mRNA and telomerase are higher than CK19 mRNA, and combined assay of the markers can improve the sensibility.
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Objective To analysis the relationship between the multipathogens infection and unhealthy pregnancy.Methods All patients was selectively divided into three groups:group A including 106 patients with unhealthy pregnancy,group B:55 gravidas with normal induced abortion,group C:55 normal parturient with term labor.The ten kinds of pathogens from abortion tissues and placentas were tested by PCR method respectively.Anti-pathogen IgM antibodies were detected by ELISA in the blood specimens.Results The distribution status of the pathogens were as followed:Human parvovirus B19(B19V 23.6%)＞Cytomegalovirus(CMV,16.0%)＞Toxoplasma (TOX，15.1%)＞Rubella virus(RV，10.4%)＞Ureaplasma urealyticum(9.4%)＞Coxsackie(7.5%)＞Herpes simplex virus(HSV,6.6%)＞Adenovirus,Eprstan Barr virus(3.8%)＞Chlamydia trachomatic(CT 2.8%) in the group A.The positive rates of the pathogens infection in both group C and group B were lower.The positive rates of B19V,CMV,RV,HSV,TOX were significant difference(χ2 test,P＝0.000～P＝0.031) among the three groups,hovever others were not(χ2 test,P＝0.121～P＝0.724).Conclusions The pathogen infection is an important factor to occur the unhealthy pregnancy,the major pathogens of unhealthy pregnancy are B19V，CMV，TOX，RV，HSV.These pathogens infection should be detected timely in gestational period.
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Chlamydia trachomatic(CT 2 8%) in the group A.The positive rates of the pathogens infection in both group C and group B were lower.The positive rates of B 19 V,CMV,RV,HSV,TOX were significant difference(? 2 test,P=0 000～P=0 031) among the three groups,hovever others were not(? 2 test,P=0 121～P=0 724).Conclusions The pathogen infection is an important factor to occur the unhealthy pregnancy,the major pathogens of unhealthy pregnancy are B 19 V,CMV,TOX,RV,HSV.These pathogens infection should be detected timely in gestational period.
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Purpose:To evaluate the differences in expression of carcinoembryonic antigen mRNA (CEA mRNA) in various tissues and its use in the diagnosis of digestive tract carcinoma.Methods:There were 35 patients with digestive tract carcinoma and 12 patients with non carcinoma disease in the study. The expression of CEA mRNA in the digestive tract tissues specimens was detected with nested reverse transcriptase polymerase chain reaction(RT PCR). A contrast test was detection of carcinoembryonic antigen (CEA) in blood sepcimen with enzyme linked immunosorbent assay (ELISA). Results:The expression of CEA mRNA in cancer tissues with RT PCR was 29 (82.86%) with positive results and that of CEA in blood with ELISA was 15 (42.86%) in the patients with digestive tract carcinoma. The difference in positive results was significant ( P
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Objective To study the practical methods for the diagnosis of clinical etiology of acute respiratory infection(ARI).Methods 108 patients with ARI were the expermental group and forty healthy peoples were the control group.The specimens from throaty excretions were collected in the both groups.Adenovirus(ADV),coxsackie virus(COX),respirator syncytial virus(RSV),chlamidia pneumonia(CP) and mycoplasma pneumonia(MP) in the specimens were detected with polymerase chain reaction(PCR).Results The detected positive rates were 10 2% for ADV,17 6% for COX,28 7% for RSV,33 3% for CP and 13 9% for MP respectively in ARI.There was significantly difference between the ARI group and the healthy control group(P
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Purpose:To determine the diagnostic value of detection of carcinoembryonic antigen mRNA (CEA mRNA), CEA and CA19-9 for diagnosis and differential diagnosis of malignant or benign hydrothorax. Methods:The groups included 76 cases of malignant hydrothorax group and 58 cases in benign hydrothorax group from whom specimens of chest fluid were obtined. CEA mRNA was detected by reverse transcriptase-nested primer-polymerase chain reaction (RT-NP-PCR). CEA and CA19-9 were detected by magnetic antibody immunoenzymetric assay (MAIA). Results:The positive rates of CEA mRNA, CEA and CA19-9 were 78.9%(60/76), 52.6%(40/76) and 55.3%(42/76) in the malignant hydrothorax group, and 8.6% (5/58), 5.2%(3/58) and 3.4%(2/58) in the benign dropsy of chest group respectively. There were significant differences in the positive results of CEA mRNA, CEA and CA19-9 between benign and malignant hydrothorax(P